OMEGA 3 FATTY ACID AND SIDER HONEY ATTENUATE SODIUM VALPROATE -INDUCED LIVER INJURY IN FEMALE ALBINO RATS

Abstract

Sodium valproate (SVP) is one of the most widely prescribed antiepileptic drugs; however, its clinical use is frequently limited by hepatotoxicity, which is primarily associated with oxidative stress and inflammatory mechanisms. This study aimed to investigate the potential protective effects of Omega 3 (O3) and Sider honey (SH) against SVP-induced liver injury in female rats. Thirty female albino rats were randomly divided into five groups (n=6): group I (control) received distilled water; group II received SVP 300mg/kg/day; group III received SVP +O-3 (300mg/kg/day); group IV received SVP +SH (5g/kg/day); and group V received SVP + O-3+ SH. All treatments were administered orally for 14 consecutive days. Serum malondialdehyde (MDA) and liver function enzymes (ALT, AST, and ALP) were assessed. The results revealed that SVP administration significantly increased serum MDA levels compared to the control group (p ˂0.004). On other hand, group III, IV and V showed a significant reduction in serum MDA compared to SVP group. The liver enzymes (ALT, AST, and ALP) also were significantly increased by SVP (p < 0.001). Co-treatment with Omega-3, Sider honey, or their combination resulted in a significant reduction in liver enzyme levels compared to the SVP-treated group (p < 0.05). The combined treatment demonstrated the most pronounced hepatoprotective effect. Omega 3 and Sider honey effectively attenuated Sodium valproate-induced hepatotoxicity, possibly through the suppression of oxidative stress and improvement of liver enzymes. These findings suggest that Omega 3 and Sider honey may serve as hepatotoxicity protective agent, warranting further clinical research.